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Position of Hfq-binding site is important for RNA annealing. (a) Scheme of target RNAs listed in Supplementary Table S1. (b) Observed rate constants (kobs) obtained from the binding kinetics between the molecular beacon and different RNA targets (left) in 1 µM Hfq monomer. Rate constants from five independent experiments were averaged and standard deviations indicated by error bars. See Figures S3–S5 for additional data.
