Figure 3.

MyoD relieves the enhancer-blocking activity of a KvDMR1 sub-region. (a) Schematic representation of the plasmid construct used for the enhancer-blocking assay, indicating the extent of the [11–22] test fragment (31) with respect to the KvDMR1 repressive element reported in Figure 2a. (b) Relative enhancer-blocking activity of the 11–22 fragment of KvDMR1 in K562 cells co-transfected with the MyoD expression vector (MyoD) or with the empty vector (Control). For each co-transfection, values, normalized relative to luciferase activity, were determined by dividing the number of G418-resistant colonies obtained with the indicated constructs by the colony number obtained with the Epneo construct. The results are the mean of three independent transfection experiments.