Figure 2.
The basic macro-linker reduces the availability of extranucleosomal DNA for Chd1 binding. (A) Mixtures nucleosomes containing major-type H2A and mH2A122 (5 nM each) were incubated with increasing amounts of the chromatin remodeler Chd1 (5, 10, 20, 40, 60 and 80 nM) for 1 h on ice and resolved on a 6% non-denaturing gel. Left image is an FAM-scan showing the locations of major-type H2A nucleosomes, and right image is a Cy5-scan showing the mH2A122 nucleosomes. (B) Similar experiment as shown in (A), except that Cy5-labeled nucleosomes contained mH2A161. These gels are representative of more than three experiments carried out under similar conditions.