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. 2012 Jun 28;40(17):8440–8448. doi: 10.1093/nar/gks646

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Caffeine and CHK1 inhibition both retards replication fork progression in Polη-deficient cells. (A) Outline of the alkaline DNA unwinding assay to monitor replication fork elongation. Unwinding starts from DNA ends such as those present at a replication fork, allowing the monitoring of the fraction of labelled DNA that ends up in the single-stranded and double-stranded fractions, respectively. (B) Replication fork progression after UVC irradiation (5 J/m²) and the presence of 1 mM caffeine in Polη-deficient XP30RO and restored cells. (C) Replication fork progression after UVC irradiation (5 J/m²) and the presence of 0.5 µM CEP-3891 in Polη-deficient XP30RO and restored cells.