Table 1.
Recent Applications of Raw Material Strategies for In Vitro Bone Tissue Engineering Applications
| Reference(s) | Raw material(s) | Additional material(s) | Scaffold formulation | Cell type | Growth factor supplementation |
|---|---|---|---|---|---|
| Chan et al.44 | Collagen type I (rat) | – | Microspheres | Human and murine MSCs | – |
| Koegh et al.46 | Collagen type I (bovine), CS | – | Porous composite | Human fetal osteoblasts | TGF-β1 |
| Kruger et al.34 | Collagen type I (bovine) | PLGA | Porous matrix | Human MSCs | – |
| Shen et al.42 | Collagen type I (porcine), HAp | – | Nanocomposite hydrogel | – | – |
| Sionkowska and Kozlowska29 | Collagen type I, HAp | – | Nanocomposite hydrogel | – | – |
| Thein-Han and Xu35 | Collagen type I (rat) | CaP cement, alginate | Injectable microbead hydrogel | Human UCMSCs | – |
| Wang and Stegemann40 | Collagen type I (bovine) | Chitosan | Composite hydrogel | Human MSCs | – |
| Akkouch et al.27 | Collagen type Ib, HAp | PLCL | Porous composite | Human osteosarcoma | HEGF |
| Chicatun et al.57 | Collagen type I (rat) | Chitosan | Dense collagen composite hydrogel | MC3T3-E1 | – |
| Marelli et al.43 | Collagen type I (rat) | Bioactive glass | Composite hydrogel | MC3T3-E1 | – |
| Bae et al.80 | HA | – | Hydrogel | MC3T3-E1 | Simvastatina |
| Chen et al.81 | HA, collagen type Ib | PCL | Porous matrix | hMSC-TERT | – |
| Chen et al.82 | HA, collagen type I (bovine) | Bioactive glass, PS | Porous composite | MC3T3-E1 | – |
| Liao et al.83 | HA | HA-CPN | Injectable, thermoresponsive hydrogel | Canine MSCs | TGF-β1 |
| Li et al.104 | HAp | Chitosan, PLLA | Porous composite | MC3T3-E1 cells | – |
| Liu et al.109 | nHAp, collagen type Ib | PLA | Porous composite | Rabbit DPSCs | BMP-2 |
| Peng et al.107 | HAp | PLLA | Nanofibrous composite | Rat osteosarcoma cells | – |
| Prosecka et al.110 | HAp, collagen type I (bovine) | – | Porous composite | Porcine MSCs | – |
| Haimi et al.122 | TCP | PLA, bioactive glass | Porous composite | Human ASCs | – |
| Lee et al.117 | TCP, collagen type I (porcine) | PCL | Porous composite | MG63 | – |
| Lin et al.131 | TCP | – | Porous matrix | – | – |
| Rai et al.118 | TCP | PCL | Porous composite | hMSCs | – |
| Yanoso-Scholl et al.123 | TCP | PLA | Porous composite | None | BMP-2, VEGFa |
| Yeo et al.120 | TCP, collagen type I (porcine) | PCL | Nanofibrous composite | MG63 | – |
| Zhang et al.126 | TCP, collagen type Ib | – | Microfibrous composite | MG63 | – |
| Honsawek et al.141 | SIS, human DBM | – | Composite matrix | Human periosteal cells | – |
| Supronowicz et al.155 | Human DBM | – | Porous matrix | Human ASPSCs | – |
| Thomas et al.160 | Bovine DBM | PL | Composite matrix | Murine MSCs | – |
| Lee et al.156 | Human DBM, HAp | – | Porous composite | Human MSCs | – |
| Liu et al.152 | pDBM | – | Porous matrix | UCB-BMSCs | TGF-β1 |
| Chen et al.153 | Bovine DBM | Heparin | Porous matrix | HUVECs | VEGFa |
| Jayasuriya et al.162 | Human DBM | PLGA | Composite film | Murine MSCs | – |
| Kang et al.158 | Human DBM | Fibrin glue | Composite glue | Porcine SDMSCs | – |
Denotes incorporation of the molecules into the scaffold. All other entries indicate the addition of the growth factor to culture medium; dexamethasone, β-glycerophosphate, and ascorbic acid were considered standard osteogenic medium components and not factored in for growth factor supplementation.
Collagen species not specified.
CS, chondroitin sulfate; HAp, hydroxyapatite; HA, hyaluronic acid; TCP, β-tricalcium phosphate; SIS, porcine small intestinal submucosa; DBM, demineralized bone matrix; pDBM, partially demineralized porcine trabecular bone; PLGA, poly(lactic-co-glycolic acid); CaP, calcium phosphate; PLCL, poly(lactide-co-ɛ-caprolactone); HA-CPN, hyaluronic acid-g-chitosan-g-poly(N-isopropylacrylamide); PCL, poly(ɛ-caprolactone); PLA, poly(L-lactic acid); PLLA, poly(L-lactic acid); PL, polylactide; MSCs, mesenchymal stem cells; UCMSCs, umbilical cord mesenchymal stem cells; MC3T3-E1, murine calvarial osteoblasts; hMSC-TERT, human mesenchymal stem cell–telomerase reverse transcriptase gene-transduced; DPSCs, dental pulp stem cells; ASCs, adipose stem cells; MG63, human osteoblast-like cells; ASPSCs, adipose-derived side population stem cells; UCB-BMSCs, human umbilical cord blood–derived mesenchymal stem cells; HUVECs, human umbilical vein endothelial cells; SDMSCs, skin-derived mesenchymal stem cell–like cells; TGF-β1, transforming growth factor beta-1; HEGF, human epidermal growth factor; BMP-2, bone morphogenetic protein-2; VEGF, vascular endothelial growth factor.