Fig 1.

Scandium susceptibility of B. subtilis mutants. B. subtilis strains 168 (wild type), SC26 (scandium-resistant mutant), and a transformant TI392 (uppS86) were grown in L medium at 37°C for 4 h. Cells (approximately 5 × 10⁸ cells/ml) were appropriately diluted with distilled water. Then, aliquots of 2 μl of cell suspension were spotted onto the scandium-free (−) and scandium-containing (+) (320 μg/ml of ScCl₃ · 6H₂O) medium, followed by incubation at 37°C for 18 h.