Table 2.
Oligonucleotides used in this study
| Primer | Oligonucleotide sequence (5′ → 3′)a |
|---|---|
| HindIII-yufQ F | CCCAAGCTTGTCCCTGCCACACTCG |
| BamHI-yufQ R | CGCGGATCCGCTGACTGTCTGGTCG |
| HindIII-bcrC F | CCCAAGCTTGTCGCCTATGCCCTTATCC |
| BamHI-bcrC R | CGCGGATCCCAAAAATGACAAGCGGC |
| yubB-F | CGGTTGCCAAAGTCATTACG |
| yubB-R | GCCTTAAGAAGAAACGGACG |
| pHASH120-uppSF | ATGCTCAACATACTCAAAAATTGGAAG |
| pHASH120-uppSR | GATCATTCCGTCGTTTTGCG |
| pHASH120-yubBF | ATGACTCTATGGGAATTGTTTGTAGC |
| pHASH120-yubBR | GTGTCTGTCGCTGTTTTCCG |
| RT-qPCR 16S-F | ATCTTCCGCAATGGACGA |
| RT-qPCR 16S-R | GCCGTGGCTTTCTGGTTA |
| RT-qPCR uppS-F | CAAACTGCGGCTTCCAAC |
| RT-qPCR uppS-R | CGGCCATTTCCATCCATG |
| RT-qPCR bcrC-F | ATACGCAGGCATCACAGG |
| RT-qPCR bcrC-R | CACCTGTCGTATGGTCAC |
| RT-qPCR yubB-F | AAATTGCCGTCGGACTCG |
| RT-qPCR yubB-R | GACCCAGTCAGCAAAAAGC |
a
HindIII and BamHI restriction sites are underlined.