Table 4.
Specific activity of TM1469 on various hexoses at 37°C and 80°C
| Substratea | Sp act (U mg−1) |
|
|---|---|---|
| 37°C | 80°C | |
| d-Glucose | 10.69 ± 4.03 (100)b | 104.9 ± 3.5 (100) |
| 2-Deoxy-d-glucose | 3.74 ± 0.52 (35) | 33 (32) |
| N-Acetyl-d-glucosamine | 0.95 ± 0.57 (8.8) | 18.68 (18) |
| d-Glucosamine | 2.29 ± 1.52 (21) | 12.13 ± 1.59 (11.5) |
| d-Mannose | 0.99 ± 0.13 (9.2) | 4.99 ± 1.41 (1.9) |
| d-Mannosamine | 0.25 (2.3) | 0.57 ± 0.36 (0.5) |
| N-Acetyl-d-mannosamine | 0.58 (5.4) | 0.082 (0.07) |
a
Substrates were used at 5 mM.
b
Data in parentheses represent percentage of total activity.