Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| V. cholerae | ||
| N16961 | Wild type, lacking hapR function, O1 serogroup, biotype El Tor, Smr | 14 |
| C6709 | Wild type, hapR+, O1 serogroup, biotype El Tor, Smr | 20 |
| N-DksA1 | N16961 ΔdksA∷kan; Kmr Smr | This study |
| C-DksA1 | C6709 ΔdksA∷kan; Kmr Smr | This study |
| NRVDK2 | N-DksA1 ΔrelV∷aadA1; Kmr Smr Spr | This study |
| E. coli | ||
| DH5α | F′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (ϕ80dlacZΔM15) | Promega |
| SM10λpir | thi thr leu tonA lacY supE recA∷RP4-2-Tc∷Mu λpir R6K | 20 |
| S17-1λpir | thi proA hsdR recA∷RP4-2-Tc∷Mu-1 kan∷Tn7 integrant λpir R6K; Tpr Smr | Lab stock |
| CF1648 | Wild type MG1655 | 54 |
| CF9240 | CF1648 ΔdksA∷Tet; Ttr | 9 |
| Plasmids | ||
| pDrive | pUC origin, high-copy-no. cloning vector; Apr Kmr | Qiagen |
| PBluescript II KS(+) | ColE1, high-copy-no. cloning vector; Apr | Stratagene |
| pKAS32 | rpsL suicide vector with oriR6K mobRP4; Apr | Lab stock |
| pBR322 | pMB1 origin, general-purpose cloning vector; Apr Ttr | Lab stock |
| pUC4K | Source of kanamycin resistance gene cassette; Apr Kmr | Pharmacia |
| pBAD24 | pBR322 origin, l-arabinose-inducible vector; Apr | Lab stock |
| pBS20 | 2.9-kb ΔrelV∷aadA1 allele in pKAS32; Apr Spr | 14 |
| pJK537 | 2.3-kb dksA region of E. coli strain MG1655 in pBR322; Apr | 9 |
| pDDKW1 | 841-bp dksA region of V. cholerae strain N16961 containing dksA gene with its putative natural promoter cloned in pDrive; Apr Kmr | This study |
| pBSDA3.5 | 0.6-kb ΔdksA allele in pBluescript II KS(+); Apr Kmr | This study |
| pBSDA4.8 | 1.8-kb ΔdksA∷kan allele in pBluescript II KS(+); Apr Kmr | This study |
| pKDK1 | 1.9-kb ΔdksA∷kan allele in pKAS32; Apr Kmr | This study |
| pDksAVc | 841- bp dksA region of V. cholerae strain N16961 from pDDKW1 subcloned pBR322; Apr | This study |
| pDksABAD | V. cholerae dksA ORF cloned in pBAD24; Apr | This study |
| pFliABAD | V. cholerae fliA ORF cloned in pBAD24; Apr | This study |
| pRelVBAD | V. cholerae relV ORF cloned in pBAD24; Apr | This study |