Figure 4.
PE-induced increase in TUNEL-positive cells (brown nuclei) and the effect of Cu addition in primary cultures of neonatal rat cardiomyocytes. Cells ere cultured in a Lab-Tek II chamber glass slide, following the same treatments described for Figure 2. The experimental procedure the data acquisition and analysis were described in the Materials and Methods. The image scale bar = Values are means ± S.E.M. *, significantly different from control group and †, significantly different from PE-treated group (p < 0.05).