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. Author manuscript; available in PMC: 2012 Sep 26.
Published in final edited form as: J Mol Cell Cardiol. 2008 Apr 9;45(1):106–117. doi: 10.1016/j.yjmcc.2008.03.022

Figure 6.

Figure 6

Flow cytometry analysis of cell mitotic activity. At the end of Cu treatment as described in Figure 1, the cells were collected, fixed, and incubated with polyclonal p-H3 (Ser10) antibody (Alexa Fluor 488 conjugate) and with PI as described in the Materials and Methods. The cells were then subjected to flow cytometry analysis and 30,000 cells in each sample were counted. Representative histograms demonstrating the percentage of p-H3 stained cells under different experimental conditions. DNA content was assessed by PI staining. Quantitative analysis of the p-H3 stained cells among the total population was shown in the bar graph. The labels for the treatment groups are the same as described in Figure 2. Data were obtained from three independent experiments and each experiment contains a triplicate samples for each treatment. Values are means ± S.E.M. *, significantly different from control group (p < 0.05).