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. 2012 Sep 26;7(9):e46086. doi: 10.1371/journal.pone.0046086

Figure 1. Steps involved in the development of the first-generation Test Cancer BioChip.

Figure 1

Shown is the first-generation Cancer BioChip (CBC-1) that is capable of testing the effect of 50 individual siRNAs on colony growth in soft agar. For this study, four CBC-1 were used for screening 40 siRNAs including positive and negative controls. After addition of base agar (step 1), the siRNA was mixed with agar and applied in each well (step 2) followed by cells that were also embedded in agar (step 3). The CBC-1 was fed with medium after a 24-hour incubation (step 4) and growth of colonies was monitored at different time points thereafter using an inverted microscope (step 5). Microscopic image analysis allows for identification of siRNAs that affected colony growth (step 6).