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. 2012 Sep 26;7(9):e46095. doi: 10.1371/journal.pone.0046095

Figure 1. Genomic structure and the presence of plasmid pSFxv_2 in serotype Xv and X strains.

Figure 1

A, genomic structure of plasmid pSFxv_2. ORFs were shown as thick arrows, and encoding regions were marked according to the genome annotation (accession No. CP001385). The specific lpt-O gene was marked in red color, and primers positions were indicated using thin arrows. B, plasmid profiles of serotype Xv strains 2002017, 2008129, 2008131, 05AH022 and 05AH027 (lanes 1–5, respectively) and X strains 06HN400, 05BJ002, 03HL001, 03HL020 and 2003055 (lanes 6–10, respectively) strains. Plasmid DNA was separated by electrophoresis with a Chef Mapper system (Bio-Rad) on a 1% SeaKem Gold agarose gel and visualized by EB staining. Plasmids isolated from strain 2002017 (lane 1) were used as control. Lambda DNA cleaved with HindIII (TaKaRa, Japan) was used as molecular mass markers. C, Southern hybridization detection of the lpt-O gene in serotype Xv strains. PCR product amplified from strain 2002017 using primer pair lpt-O-2 was prepared as probe.