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. 2012 Sep 26;7(9):e45895. doi: 10.1371/journal.pone.0045895

Figure 1. Identification of candidate APC/C substrates by protein expression profiling.

Figure 1

(A) Analysis of protein levels in G1 and M. Cells expressing the indicated TAP-tag fusion proteins were arrested in G1 or M phase and the corresponding cell extracts were immunoblotted with PAP antibodies to detect TAP-tagged proteins. (B) Protein degradation in G1 and M. Cells were arrested in either G1 or M phase and treated with 500 µg/ml cycloheximide for the indicated times. Cell extracts were prepared and immunoblotted to examine TAP-tag protein levels as in (A).