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. 2012 Sep 26;7(9):e45895. doi: 10.1371/journal.pone.0045895

Figure 4. Cdh1 bead binding assay for TAP-tagged fusion proteins from whole cell extracts.

Figure 4

(A) Tos4 and Pdr3 interact with wild-type Cdh1 but not with Cdh1-D12 in the yeast two-hybrid system. (B) A Cdh1-bead binding assay. An extract from cells expressing Clb2-TAP was incubated with the indicated beads and binding was detected using PAP antibodies. WCE, whole cell extract. (C) Left panel: Extracts of cells expressing the indicated TAP-tag fusion proteins were incubated with Cdh1 beads or control beads and the levels of bound TAP-tagged proteins were detected by blotting with PAP antibodies (lanes, 1–10). Right panel: 2% of the input material (whole cell extract, WCE) for the binding assays is shown (lanes 11–15).