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Binding of OmpC with Cy3 labeled Skp mutants measured by stopped-flow kinetics experiment. (A–C) Fluorescence time-course monitoring the entrance of OmpC into the Cy3 dye labeled Skp mutants (Skp-K55C-Cy3, Skp-E82C-Cy3, and Skp-D128C-Cy3). The data were fitted to functions containing two- or three- exponential terms. (D) Comparison of the static fluorescence intensity of Cy3 dye labeled Skp mutants in the presence and absence of OmpC.
