Table 1. Natural transformation efficiency of C. jejuni NCTC11168.
CFU:DNA | CFU/mla | DNAb/ml | Transf./CFUac | Transf./DNA moleculead |
ca. 1∶10e | 3.0×108±2.9×108 | 6500 ng ≈ 3.5×109 DNA molecules | 3.6×10−3±1.8×10−3 | − |
ca. 10∶1f | 3.6×108±2.6×107 | 65 ng ≈ 3.5×107 DNA molecules | − | 5.4×10−3±1.0×10−3 |
Results are mean of three replicates with standard deviation.
Chromosomal DNA from C. jejuni NCTC11168 Δtlp1::CamR.
Limits of range tested: 10−4–100 transformants/CFU.
Limits of range tested: 10−5–10−1 transformants/DNA molecule.
Excess amounts of DNA.
Limited amounts of DNA.