Table 3. Natural transformation of C. jejuni at varying temperature and atmospheric conditionsa,b.
Temperature | Oxygen level | ||
Aerobic | Microaerobic | Anaerobic | |
20°C | 0.11±0.13 | 0.16±0.27 | Nd.c |
30°C | 15.1±8.92 | 19.6±7.37 | Nd.c |
37°C | 97.3±10.3 | Index 100b ±55.5 | 28.1±12.9 |
42°C | 130±50.8 | 353±37.8 | 16.2±6.49 |
Exponentially growing cells were supplied with isogenic chromosomal DNA at 1∶2.5 ratio (CFU:DNA) and transformation was allowed to proceed for 4 h before plating on selective agar. Results are the average of three independent experiments with two replicates.
Results are normalized to the number of transformants per ml at 37°C under microaerophilic conditions.
Not determined.