Table 4. Natural transformation is dependent on active electron transport.
Respiratory inhibitor | Time of addition | CFU/mlc | Transformants/CFUc |
Negative control | – | Index 100 | Index 100 |
HQNOd | During transformationb | 88 | 0.71 |
HQNOd | Prior to transformationa | 57 | 25 |
Sodium azidee | During transformationb | 65 | 0.61 |
Sodium azidee | Prior to transformationa | 64 | 4.5 |
Exponentially growing cells were incubated with the respiratory inhibitor for 2 h prior to a wash out of compound and addition of DNA.
The respiratory inhibitor were added to the exponentially growing cells together with the DNA.
Normalized to the negative control.
100 µg/ml.
500 µM.