Skip to main content
. 2012 Sep 26;7(9):e45467. doi: 10.1371/journal.pone.0045467

Table 4. Natural transformation is dependent on active electron transport.

Respiratory inhibitor Time of addition CFU/mlc Transformants/CFUc
Negative control Index 100 Index 100
HQNOd During transformationb 88 0.71
HQNOd Prior to transformationa 57 25
Sodium azidee During transformationb 65 0.61
Sodium azidee Prior to transformationa 64 4.5
a

Exponentially growing cells were incubated with the respiratory inhibitor for 2 h prior to a wash out of compound and addition of DNA.

b

The respiratory inhibitor were added to the exponentially growing cells together with the DNA.

c

Normalized to the negative control.

d

100 µg/ml.

e

500 µM.