Table 1. Sequence alignment of CDRs 1–2 and FRs 1–3 from D9 VH with human germline HC V genes.
| FR1 | CDR1 | FR2 | CDR2 | FR3 | |
| ·········10········20········30 | ····· | ····40········ | 50·a·······60···· | ····70········80·abc·······90··· | |
| ·········|·········|·········| | ····· | ····|········· | |··········|····· | ····|·········|············|···· | |
| D9 | QVQLQESGPELVKPGASMKISCKASGYSFT | EHIIN | WVKQTHRENLEWIG | LINPNSGGTNYNQKFKD | KATLTVDTASNTAYMELLSLTSEDSAVYYCAR |
| ·*?*·················*····**** | ····· | ****····****** | ················· | ******·*····*····*··········**** | |
| 1–18 | QVQLVQSGAEVKKPGASVKVSCKASGYTFT | SYGIS | WVRQAPGQGLEWMG | WISAYNGNTNYAQKLQG | RVTMTTDTSTSTAYMELRSLRSDDTAVYYCAR |
| 1–69 | QVQLVQSGAEVKKPGSSVKVSCKASGGTFS | SYAIS | WVRQAPGQGLEWMG | GIIPIFGTANYAQKFQG | RVTITADESTSTAYMELSSLRSEDTAVYYCAR |
| 1-e | QVQLVQSGAEVKKPGSSVKVSCKASGGTFS | SYAIS | WVRQAPGQGLEWMG | GIIPIFGTANYAQKFQG | RVTITADKSTSTAYMELSSLRSEDTAVYYCAR |
| 1-f | EVQLVQSGAEVKKPGATVKISCKVSGYTFT | DYYMH | WVQQAPGKGLEWMG | LVDPEDGETIYAEKFQG | RVTITADTSTDTAYMELSSLRSEDTAVYYCAT |
| 5–51 | EVQLVQSGAEVKKPGESLKISCKGSGYSFT | SYWIG | WVRQMPGKGLEWMG | IIYPGDSDTRYSPSFQG | QVTISADKSISTAYLQWSSLKASDTAMYYCAR |
| 5-a | EVQLVQSGAEVKKPGESLRISCKGSGYSFT | SYWIS | WVRQMPGKGLEWMG | RIDPSDSYTNYSPSFQG | HVTISADKSISTAYLQWSSLKASDTAMYYCAR |
| 7–4·1 | QVQLVQSGSELKKPGASVKVSCKASGYTFT | SYAMN | WVRQAPGQGLEWMG | WINTNTGNPTYAQGFTG | RFVFSLDTSVSTAYLQICSLKAEDTAVYYCAR |
Note: Key residues in FRs are marked with *.