Table 2. Sequence alignment of CDRs 1–2 and FRs 1–3 from D9 VL with human germline LC V genes.
| FR1 | CDR1 | FR2 | CDR2 | FR3 | CDR3 | |
| ·········10········20·· | ······30··· | ·····40········ | 50····· | ···60········70········80······· | ·90···· | |
| ·········|·········|··· | ······|···· | ·····|········· | |······ | ···|·········|·········|········ | ·|····· | |
| D9 | DIELTQSHKFMSTSVGDRVSITC | KASQDVTAAVA | WYQQKPGQSPKLLIH | SASYRYT | GVPDRFTGSGSGSDFTFTISSVQAEDLAVYYC | QQYYNTP |
| *****················** | ··········· | **·*···******** | ······· | ****·*·********···············** | ······· | |
| O12 | DIQMTQSPSSLSASVGDRVTITC | RASQSISSYLN | WYQQKPGKAPKLLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQSYSTP |
| O2 | DIQMTQSPSSLSASVGDRVTITC | RASQSISSYLN | WYQQKPGKAPKLLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQSYSTP |
| O18 | DIQMTQSPSSLSASVGDRVTITC | QASQDISNYLN | WYQQKPGKAPKLLIY | DASNLET | GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC | QQYDNLP |
| O8 | DIQMTQSPSSLSASVGDRVTITC | QASQDISNYLN | WYQQKPGKAPKLLIY | DASNLET | GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC | QQYDNLP |
| A20 | DIQMTQSPSSLSASVGDRVTITC | RASQGISNYLA | WYQQKPGKVPKLLIY | AASTLQS | GVPSRFSGSGSGTDFTLTISSLQPEDVATYYC | QKYNSAP |
| A30 | DIQMTQSPSSLSASVGDRVTITC | RASQGIRNDLG | WYQQKPGKAPKRLIY | AASSLQS | GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC | LQHNSYP |
| L14 | NIQMTQSPSAMSASVGDRVTITC | RARQGISNYLA | WFQQKPGKVPKHLIY | AASSLQS | GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC | LQHNSYP |
| L1 | DIQMTQSPSSLSASVGDRVTITC | RASQGISNYLA | WFQQKPGKAPKSLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQYNSYP |
| L15 | DIQMTQSPSSLSASVGDRVTITC | RASQGISSWLA | WYQQKPEKAPKSLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQYNSYP |
| L4 | AIQLTQSPSSLSASVGDRVTITC | RASQGISSALA | WYQQKPGKAPKLLIY | DASSLES | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQFNSYP |
| L18 | AIQLTQSPSSLSASVGDRVTITC | RASQGISSALA | WYQQKPGKAPKLLIY | DASSLES | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQFNSYP |
| L5 | DIQMTQSPSSVSASVGDRVTITC | RASQGISSWLA | WYQQKPGKAPKLLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQANSFP |
| L19 | DIQMTQSPSSVSASVGDRVTITC | RASQGISSWLA | WYQQKPGKAPKLLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | QQANSFP |
| L8 | DIQLTQSPSFLSASVGDRVTITC | RASQGISSYLA | WYQQKPGKAPKLLIY | AASTLQS | GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC | QQLNSYP |
| L23 | AIRMTQSPFSLSASVGDRVTITC | WASQGISSYLA | WYQQKPAKAPKLFIY | YASSLQS | GVPSRFSGSGSGTDYTLTISSLQPEDFATYYC | QQYYSTP |
| L9 | AIRMTQSPSSFSASTGDRVTITC | RASQGISSYLA | WYQQKPGKAPKLLIY | AASTLQS | GVPSRFSGSGSGTDFTLTISCLQSEDFATYYC | QQYYSYP |
| L11 | AIQMTQSPSSLSASVGDRVTITC | RASQGIRNDLG | WYQQKPGKAPKLLIY | AASSLQS | GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC | LQDYNYP |
| L12 | DIQMTQSPSTLSASVGDRVTITC | RASQSISSWLA | WYQQKPGKAPKLLIY | DASSLES | GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC | QQYNSYS |
| L2 | EIVMTQSPATLSVSPGERATLSC | RASQSVSSNLA | WYQQKPGQAPRLLIY | GASTRAT | GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC | QQYNNWP |
| L16 | EIVMTQSPATLSVSPGERATLSC | RASQSVSSNLA | WYQQKPGQAPRLLIY | GASTRAT | GIPARFSGSGSGTEFTLTISSLQSEDFAVYYC | QQYNNWP |
| L6 | EIVLTQSPATLSLSPGERATLSC | RASQSVSSYLA | WYQQKPGQAPRLLIY | DASNRAT | GIPARFSGSGSGTDFTLTISSLEPEDFAVYYC | QQRSNWP |
| L20 | EIVLTQSPATLSLSPGERATLSC | RASQGVSSYLA | WYQQKPGQAPRLLIY | DASNRAT | GIPARFSGSGPGTDFTLTISSLEPEDFAVYYC | QQRSNWH |
| B2 | ETTLTQSPAFMSATPGDKVNISC | KASQDIDDDMN | WYQQKPGEAAIFIIQ | EATTLVP | GIPPRFSGSGYGTDFTLTINNIESEDAAYYFC | LQHDNFP |
| A26 | EIVLTQSPDFQSVTPKEKVTITC | RASQSIGSSLH | WYQQKPDQSPKLLIK | YASQSFS | GVPSRFSGSGSGTDFTLTINSLEAEDAATYYC | HQSSSLP |
| A10 | EIVLTQSPDFQSVTPKEKVTITC | RASQSIGSSLH | WYQQKPDQSPKLLIK | YASQSFS | GVPSRFSGSGSGTDFTLTINSLEAEDAATYYC | HQSSSLP |
| A14 | DVVMTQSPAFLSVTPGEKVTITC | QASEGIGNYLY | WYQQKPDQAPKLLIK | YASQSIS | GVPSRFSGSGSGTDFTFTISSLEAEDAATYYC | QQGNKHP |
Note: Key residues in FRs are marked with *.