Table 5. Different key residues of FRs between human donors and D9.

Position		D9	Human donors	Favored or neutral substitution
VH	28	S	T	Yes (small, polar)
	38	K	R	Yes (polar, positive)
	44	N	G	No
	48	I	M	Yes (polar)
	66	K	R	Yes (polar, positive)
	67	A	V	Yes (polar)
	69	L	M	Yes (polar)
	71	V	T	Yes (polar)
	82a	L	R	No
VL	3	E	Q	Yes (polar)
	4	L	M	Yes (non-polar)
	42	Q	K	Yes (polar)
	43	S	A	Yes (tiny)
	49	H	Y	Yes (polar, aromatic)
	60	D	S	Yes (small, polar)
	69	S	T	Yes (small, polar)
	100	A	G	Yes (tiny, non-polar)