Table 1.
qPCR analysis for hepatic markers in differentiated UCMSCs
| 7 days | *p | 14 days | *p | 21 days | *p | 28 days | *p | ¥p | |
|---|---|---|---|---|---|---|---|---|---|
|
Petri dishes | |||||||||
| AFP |
0.481 |
0.0402 |
0.573 |
0.0377 |
0.358 |
0.124 |
0.0003 |
0.0378 |
|
| (0.472; 0.482) |
(0.566; 0.575) |
(0.357; 0.364) |
(0.122; 0.124) |
(z = −2.08) |
|||||
| Alb |
2.652 |
4.298 |
5.991 |
7.880 |
0.0219 |
||||
| (2.630; 2.683) |
(4.276; 4.438) |
(5.966; 6.261) |
(7.718; 8.144) |
(z = 2.30) |
|||||
| MTP |
1.012 |
NS |
1.924 |
3.606 |
4.805 |
0.0021 |
|||
| (1.007; 1.017) |
(1.910; 1.929) |
(3.521; 3.612) |
(4.741; 4.821) |
(z = 3.08) |
|||||
| TDO |
7.464 |
10.483 |
16.111 |
19.575 |
0.0023 |
||||
| (7.380; 7.576) |
(10.301; 10.601) |
(16.040; 16.295) |
(19.497; 19.802) |
(z = 3.12) |
|||||
|
MatrigelTM | |||||||||
| AFP |
0.719 |
0.0450 |
0.592 |
NS |
0.638 |
0.0202 |
0.587 |
NS |
|
| (0.715; 0.719) |
(0.591; 0.596) |
(0.635; 0.639) |
(0.583; 0.589) |
||||||
| Alb |
0.704 |
1.437 |
NS |
1.926 |
NS |
2.486 |
0.0065 |
||
| (0.701; 0.704) |
(1.419; 1.445) |
(1.895; 1.945) |
(2.446; 2.520) |
(z = 2.72) |
|||||
| MTP |
0.789 |
NS |
0.814 |
NS |
2.452 |
2.274 |
0.0122 |
||
| (0.781; 0.790) |
(0.813; 0.820) |
(2.432; 2.461) |
(2.258; 2.278) |
(z = 2.51) |
|||||
| TDO |
1.250 |
NS |
1.210 |
NS |
1.269 |
NS |
1.490 |
NS |
NS |
| (1.170; 1.317) |
(1.140; 1.374) |
(1.258; 1.428) |
(1.443; 1.594) |
||||||
|
hLAM | |||||||||
| AFP |
0.992 |
NS |
0.823 |
NS |
0.778 |
NS |
0.871 |
NS |
NS |
| (0.982; 0.994) |
(0.819; 0.829) |
(0.777; 0.788) |
(0.837; 0.876) |
||||||
| Alb |
1.580 |
0.0353 |
0.860 |
NS |
0.834 |
NS |
0.788 |
NS |
NS |
| (1.570; 1.595) |
(0.859; 0.867) |
(0.822; 0.847) |
(0.786; 0.793) |
||||||
| MTP |
nd |
|
nd |
|
nd |
|
nd |
|
|
| TDO | nd | nd | nd | nd | |||||
*p Mann–Whitney U-test ¥p Cuzick’s trend test.
Values represent median (Q1; Q3) of 5 independent experiments. Data were analyzed using the second-derivative algorithm. For each sample, the quantity of specific hepatic marker mRNA was expressed as n-fold the normalized amount of mRNA from undifferentiated control cells.
The Mann–Whitney U-test was used for the statistical analysis of differences between undifferentiated control cells and differentiated cells at each time points (*p). Multiple comparisons to evaluate the changes of a single mRNA expression during the time were performed with the Cuzick’s trend test (¥p). nd: not detected; NS: not significant.