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. 2012 Jul 12;12:88. doi: 10.1186/1471-230X-12-88

Table 3.

Characterization of UCMSCs after 28 days of culture

  Undifferentiated UCMSCs Differentiated UCMSCs
MSC Markers
 
 
CD29
53.61%
79.4%
CD73
97.3%
78.8%
CD90
43.15%
32.8%
CD105
72.6%
13.7%
CD166
61.5%
96.5%
HSC Markers
CD14
0%
0%
CD34
0.7%
7.3%
CD45
1.2%
1.3%
CD71
10.4%
4.5%
c-kit
0%
5.8%
HLA-DR 0% 0%

Flow cytometry analysis of differentiated UCMSCs on untreated plastic support after 28 days showed a lower expression of the typical MSC markers, such as CD73, CD90 and CD105. CD166 was spontaneously lost in undifferentiated cells whereas its expression was maintained in differentiated UCMSCs. CD29 expression in differentiated cells increased to reach a level comparable with human hepatocytes [7]. Hematopoietic markers remained stable for up to 28 days both in differentiated and untreated cells.