Table 3.
Characterization of UCMSCs after 28 days of culture
| Undifferentiated UCMSCs | Differentiated UCMSCs | |
|---|---|---|
|
MSC Markers |
|
|
| CD29 |
53.61% |
79.4% |
| CD73 |
97.3% |
78.8% |
| CD90 |
43.15% |
32.8% |
| CD105 |
72.6% |
13.7% |
| CD166 |
61.5% |
96.5% |
|
HSC Markers | ||
| CD14 |
0% |
0% |
| CD34 |
0.7% |
7.3% |
| CD45 |
1.2% |
1.3% |
| CD71 |
10.4% |
4.5% |
| c-kit |
0% |
5.8% |
| HLA-DR | 0% | 0% |
Flow cytometry analysis of differentiated UCMSCs on untreated plastic support after 28 days showed a lower expression of the typical MSC markers, such as CD73, CD90 and CD105. CD166 was spontaneously lost in undifferentiated cells whereas its expression was maintained in differentiated UCMSCs. CD29 expression in differentiated cells increased to reach a level comparable with human hepatocytes [7]. Hematopoietic markers remained stable for up to 28 days both in differentiated and untreated cells.