Figure 1. Characterization of MEF^PTEN−/−.

(A) Western blot analyses of PTEN, Akt, p-Ser⁴⁷³Akt and p-Thr³⁰⁸Akt Immunoblotting of PTEN, Akt, p-Ser⁴⁷³Akt and p-Thr³⁰⁸Akt was carried out on whole cell lysates of MEF^WT and MEF^PTEN−/−. The loss of PTEN in MEF^PTEN−/− resulted in hyperactivation of Akt on both phosphorylation sites without affecting the expression of total Akt. The respective loading controls of α-tubulin were included. (B) Rate of cell proliferation This was measured by the trypan-blue exclusion assay. The MEF^PTEN−/− has a significantly higher rate of proliferation compared to MEF^WT from days 1–4 of culture. # p<0.005 for n = 3.