Figure 5. Transcriptional Regulation.

(A) Western blots of mitochondrial transcription factors The protein expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), nuclear respiratory factor-1 (NRF-1) and mitochondrial DNA transcription factor A (mtTFA) were measured in MEF^WT and MEF^PTEN−/−. Quantitative analysis showed no significant difference between the two (inset). (B) Measurement of mRNA by real-time PCR The mRNAs of the five subunits RCs used in our Western blot analysis were not significantly different in MEF^PTEN−/− and MEF^WT with p values shown in the accompanying table (inset). Mouse alpha 1A tubulin was used for normalization.