Table 3.

Strains and plasmids used in this study

Strains or plasmids	Characteristics	Reference or source
Strains
SC-19	Virulent Chinese S. suis serotype 2 isolate, wild-type	This work
ΔperR	Gene perR inactive strain, Ermr	This work
CΔperR	Complemented ΔperR strain, Ermr Spcr	This work
Δdpr	Gene dpr inactive strain, Spcr	This work
ΔperRΔdpr	Gene perR and dpr inactive strain, Ermr Spcr	This work
SC-19:EGFP	Pdpr-EGFP fusion inserted Wild-type, Spcr	This work
ΔperR:EGFP	Pdpr-EGFP fusion inserted ΔperR, Spcr	This work
DH5αand BL21(DE3)	Cloning and expression host	In this lab
Plasmids
pSET4s	Thermosensitive allelic replacement vector	[42]
pSET2	E. coli-S. suis shuttle vector	[44]
pET28a	His tag fusion expression vector	Novagen
pMIDG310	A plasmid containing a EGFP gene
pSET4s:: perR	A mosaic plasmid designed to inactivate perR	This work
pSET4s::dpr	A mosaic plasmid designed to inactivate dpr	This work
pSET2::CperR	Recombinant plasmid used for functional complementation of ΔperR	This work
pSET4s:EGFP	Recombinant plasmid used for inserting a Pdpr-EGFP fusion	This work
pET28a:: perR	Recombinant expression plasmid to produce His6-fused PerR protein	This work
pAT18	A plasmid containing an erm	[45]