Strains
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|
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SC-19
|
Virulent Chinese S. suis serotype 2 isolate, wild-type
|
This work
|
ΔperR
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Gene perR inactive strain, Ermr
|
This work
|
CΔperR
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Complemented ΔperR strain, Ermr Spcr
|
This work
|
Δdpr
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Gene dpr inactive strain, Spcr
|
This work
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ΔperRΔdpr
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Gene perR and dpr inactive strain, Ermr Spcr
|
This work
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SC-19:EGFP
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Pdpr-EGFP fusion inserted Wild-type, Spcr
|
This work
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ΔperR:EGFP
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Pdpr-EGFP fusion inserted ΔperR, Spcr
|
This work
|
DH5αand BL21(DE3)
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Cloning and expression host
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In this lab
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Plasmids
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pSET4s
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Thermosensitive allelic replacement vector
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[42]
|
pSET2
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E. coli-S. suis shuttle vector
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[44]
|
pET28a
|
His tag fusion expression vector
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Novagen
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pMIDG310
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A plasmid containing a EGFP gene
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|
pSET4s:: perR
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A mosaic plasmid designed to inactivate perR
|
This work
|
pSET4s::dpr
|
A mosaic plasmid designed to inactivate dpr
|
This work
|
pSET2::CperR
|
Recombinant plasmid used for functional complementation of ΔperR
|
This work
|
pSET4s:EGFP
|
Recombinant plasmid used for inserting a Pdpr-EGFP fusion
|
This work
|
pET28a:: perR
|
Recombinant expression plasmid to produce His6-fused PerR protein
|
This work
|
pAT18 |
A plasmid containing an erm
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[45] |