Table 4.
Primers used in this study
| Primers | Sequence | Restriction site | target |
|---|---|---|---|
| General PCR amplification | |||
| 310 F |
CGTACAGTCGACTTAGTTCTGGCAATCAGGACA |
SalI |
perR |
| 310R |
CGTATCGGATCCATGGAACTCCATTCTCACTTC |
BamHI |
|
| 310 L01: |
TAGTAAGCTTCACAGTTGGACCTTGGTT |
Hind III |
Left arm of perR |
| 310 L02 |
TCACCTGCAGCGGCATTTGTCCTGATTG |
PstI |
|
| 310R01 |
TCACCTGCAGTTAGCATTGAAGTGAGAATGG |
PstI |
Right arm of perR |
| 310R02 |
AGGTGAATTCTTGCTACTGTAATGGTCG |
EcoRI |
|
| ermF |
TCACCTGCAGGAGTGTGTTGATAGTGCA |
PstI |
ermr |
| ermR |
AGGTCTGCAGCTTGGAAGCTGTCAGTAG |
PstI |
|
| C310F |
TCACCTGCAGGATGATGTGGCTGTGTTG |
PstI |
perR and its promoter |
| C310R |
TAGTGGATCCAAGTCATGTCCGTCGTAG |
BamHI |
|
| PdprF |
TCAGAATTCTCGGGCTATAGGTAAAAG |
EcoRI |
Promoter of dpr |
| PdprR |
TCAGGATCCATATCACCCTTTCTTTTATT |
BamHI |
|
| EGFP 01 |
TCAGGATCCATGAGTAAAGGAGAAGAAC |
BamHI |
EGFP gene |
| EGFP 02 |
TCACTGCAGTGCTATTTGTATAGTTCATC |
PstI |
|
| 1772P01 |
TCCAGGACTGGTGGCGAC |
|
Promoter of 1772 |
| 1772P02 |
AAAATGATCTCCTTAAATTA |
|
|
| relAP01 |
CATATCTCTACTCTTCCTC |
|
Promoter of relA |
| relAP02 |
AGCTAGTGTGAGTGCTAC |
|
|
| gidAP01 |
CATGTTGTTCTCTCCTTC |
|
Promoter of gidA |
| gidAP02 |
TTGAGGTCAATGAGGTAG |
|
|
| Real-time RT-PCR |
|
|
|
| 0309 F |
GCAACACTTTCTGCCATCA |
|
pmtA |
| 0309R |
GGTCGCACCTACAACTTCA |
|
|
| 1771 F |
CGCACCAATCCGTCTTTA |
|
metQ |
| 1771R |
TTTCGTTTGTTGGGTCGT |
|
|
| 2094 F |
TAAGACCGACGAATCCC |
|
relA |
| 2094R |
TCATCCGCGACAGCT |
|
|
| 1689 F |
TTTATCAGTAGCCCATTCA |
|
dpr |
| 1689R |
AAACGCTCACTCATCTCA |
|
|
| 1539 F |
AGAAGGCAAGTTGGAAG |
|
sodA |
| 1539R |
GTAGTTTGGACGGACATT |
|
|
| 0155 F |
AGAAGTAAACGCTGCTAT |
|
gapdh |
| 0155R | CAAACAATGAACCGAAT | ||