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. 2012 Jul 23;9:181. doi: 10.1186/1742-2094-9-181

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Copyright ©2012 Wei et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunostaining for keratin in the hindpaw skin four weeks post-fracture. Panels exhibit confocal images from the hindpaw skin of a normal control rat, the ipsilateral hindpaw skin of a fracture rat (FX-IPSI), the contralateral hindpaw skin of a fracture rat (FX-CONTRA), and the ipsilateral hindpaw skin of a fracture rat treated with an NK1 receptor antagonist (LY303870 20 mg/kg, i.p. daily for 8 days, FX + LY-IPSI), showing staining for keratin (green). Scale bar = 25 μm. There was an increase in epidermal thickness, indicating increased keratinocyte proliferation, at 4 weeks post-fracture in the fracture paw (FX-IPSI), but not in the contralateral paw (FX-CONTRA), and this increase was blocked in fractured rats treated with LY303870 (FX + LY-IPSI).