A, key autophagy mediator beclin 1 in aorta of young and old control (YC and OC) and young and old trehalose supplemented (YTre and OTre) mice. B, p62, a marker of undegraded autophagy substrates. C and D, WIPI-1 and LC3-II, markers/indexes of macroautophagy. E, LAMP-2a, critical mediator of chaperone-mediated autophagy. Data expressed relative to GAPDH and normalized to YC mean value. Representative Western blot images below. Values are means ± SEM (n= 5–7 per group). *P < 0.05 vs. YC.