Table 2.
Smooth muscle and endothelial function in arteries
| Non-pregnant Control | Non-pregnant Restricted | |||||||
|---|---|---|---|---|---|---|---|---|
| Arteries | Uterine | Mesenteric | Renal | Femoral | Uterine | Mesenteric | Renal | Femoral |
| Smooth muscle function | ||||||||
| 100 mmol l−1 K+ (mN mm−1) | 10.5 ± 0.2 | 7.0 ± 0.4 | 11.2 ± 0.6 | 13.4 ± 0.7 | 11.3 ± 0.3 | 6.5 ± 0.3 | 10.6 ± 0.3 | 14.1 ± 0.6 |
| Maximum PE (mN mm−1) | 11.3 ± 0.2 | 6.5 ± 0.3 | 11.5 ± 0.6 | 13.4 ± 0.5 | 11.8 ± 0.4 | 6.2 ± 0.3 | 10.9 ± 0.3 | 14.1 ± 0.8 |
| Maximum Ang II (mN mm−1) | 5.5 ± 0.6 | 0.02 ± 0.01 | 0.04 ± 0.02 | 0.02 ± 0.01 | 5.5 ± 0.4 | 0.03 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.03 |
| Ang II, Emax (% K+) | 55 ± 6 | — | — | — | 49 ± 4 | — | — | — |
| PE, Emax (% K+) | 108 ± 2 | 93 ± 3 | 103 ± 1 | 101 ± 2 | 105 ± 2 | 95 ± 2 | 103 ± 2 | 99 ± 2 |
| SNP, Emax (%) | 96 ± 1 | 97 ± 1 | 88 ± 2 | 86 ± 2 | 93 ± 1 | 95 ± 1 | 89 ± 2 | 81 ± 4 |
| LEV, Emax (%) | 95 ± 1 | 91 ± 1 | 80 ± 4 | 85 ± 7 | 94 ± 1 | 97 ± 1 | 85 ± 3 | 89 ± 3 |
| Endothelium-dependent relaxation | ||||||||
| ACh, Emax (%) | 96 ± 1 | 99 ± 1 | 96 ± 1 | 90 ± 2 | 95 ± 1 | 98 ± 1 | 94 ± 1 | 90 ± 2 |
| ACh+l-NAME+Indo, Emax (%) | 34 ± 9 | 95 ± 1 | 90 ± 1 | — | 53 ± 10 | 94 ± 2 | 87 ± 2 | — |
| Pregnant Control | Pregnant Restricted | |||||||
|---|---|---|---|---|---|---|---|---|
| Arteries | Uterine | Mesenteric | Renal | Femoral | Uterine | Mesenteric | Renal | Femoral |
| Smooth muscle function | ||||||||
| 100 mmol l−1 K+ (mN mm−1) | 8.5 ± 0.5 # | 6.7 ± 0.5 | 9.3 ± 0.6 | 11.0 ± 0.9 # | 8.9 ± 0.5 # | 6.0 ± 0.5 | 10.9 ± 0.8 | 13.1 ± 0.9 # |
| Maximimum PE (mN mm−1) | 10.3 ± 0.6 # | 6.4 ± 0.5 | 9.5 ± 0.6 | 11.1 ± 1.2 # | 10.2 ± 0.5 # | 6.4 ± 0.5 | 10.3 ± 0.7 | 12.5 ± 0.8 # |
| Maximum Ang II (mN mm−1) | 8.2 ± 1.0 # | 0.06 ± 0.05 | 0.1 ± 0.04 | 0.04 ± 0.04 | 8.2 ± 0.9 # | 0.09 ± 0.06 | 0.1 ± 0.05 | 0.09 ± 0.08 |
| Ang II, Emax (% K+) | 88 ± 4 # | — | — | — | 83 ± 3 # | — | — | — |
| PE, Emax (% K+) | 122 ± 2 # | 94 ± 3 | 104 ± 4 | 100 ± 2 | 114 ± 1 # | 105 ± 3 | 95 ± 2 | 94 ± 4 |
| SNP, Emax (%) | 99 ± 1 | 97 ± 2 | 92 ± 2 | 81 ± 8 | 95 ± 1 | 98 ± 2 | 86 ± 2 | 79 ± 5 |
| LEV, Emax (%) | 91 ± 1 | 97 ± 1 | 84 ± 4 | 93 ± 1 | 87 ± 2 | 94 ± 1 | 75 ± 5 | 77 ± 7 |
| Endothelium-dependent relaxation | ||||||||
| ACh, Emax (%) | 99 ± 0.4 | 100 ± 0.2 | 96 ± 1 | 94 ± 3 | 98 ± 1 | 100 ± 0.4 | 93 ± 1 | 94 ± 5 |
| ACh+l-NAME+Indo, Emax (%) | 86 ± 2 # | 98 ± 1 | 89 ± 2 | — | 83 ± 2 # | 92 ± 2 | 86 ± 2 | — |
Responses in arteries from non-pregnant and pregnant (E20) animals. Values are expressed as mean ± SEM; uterine (n = 8–10/group); mesenteric (n = 6–10/group); renal (n = 8–10/group); femoral (n = 6–10/group). Emax, maximal response. #P < 0.05 vs. non-pregnant (main effect). — indicates no response.