Fig. 1.

HSL is deleted specifically in the small intestine of HSLiKO mice. (A) HSL mRNA levels were determined in all 3 parts of the small intestine (duodenum, jejunum, ileum) and in control tissues. mRNA levels were normalized to cyclophilin A as a reference gene. HSL mRNA levels in control mice were arbitrarily set to 1. Values represent means (n = 3) ± SEM. **p ≤ 0.01, ***p ≤ 0.001. (B) Protein lysates of pools from 3 mice of each genotype were separated by SDS-PAGE. HSL protein expression was analyzed by Western blotting. The expression of β-actin was determined as loading control. (C) CE, (D) acylglycerol and (E) retinyl ester hydrolase activities were measured in duodenum, jejunum and ileum. Data represent mean values (n = 4) ± SEM. **p ≤ 0.01, ***p ≤ 0.001.