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. 2012 Aug 22;32(34):11716–11726. doi: 10.1523/JNEUROSCI.1942-12.2012

Figure 2.

Figure 2.

BRAG1 mutants localize to excitatory synapses in hippocampal neurons. A, Dissociated rat hippocampal neurons were fixed at DIV 21, and coimmunostained with rabbit polyclonal anti-BRAG1 and mouse anti-PSD-95 antibodies. B–F, Hippocampal neurons were transfected (DIV 6) with the indicated mCherry-tagged constructs and fixed (DIV 21) for epifluorescence imaging. Cells expressing mCherry (B), mCherry-BRAG1-WT (C), mCherry-BRAG1-EK (D), mCherry-BRAG1-IQ (E), and mCherry-BRAG1-ΔN (F) were costained with PSD-95 antibody to detect excitatory synapses. Scale bars: 10 μm.