Expression of BRAG1 does not alter the basic membrane properties of CA1 neurons. A, Two-photon fluorescence imaging indicates that mCherry-BRAG1 is expressed in the dendrites and spines (inset) of CA1 pyramidal neurons. B, Evoked responses to step depolarizing and hyperpolarizing pulses recorded from neighboring nonexpressing (Ctrl) and BRAG1-expressing cells. C, Resting membrane potentials (Ctrl: −58.1 ± 1.1 mV; BRAG1: −57.2 ± 0.9 mV; n = 20; p = 0.25), input resistances (Ctrl: 189.8 ± 8.2 MΩ; BRAG1: 190.2 ± 13.2 MΩ; n = 20; p = 0.50), and time constants (Ctrl: 27.3 ± 1.7 ms; BRAG1: 28.5 ± 2.1 ms; n = 20; p = 0.97) in mCherry-BRAG1-expressing cells are plotted against those obtained from control nonexpressing cells. Blue dots indicate the average points.