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. 2012 Aug 22;32(34):11716–11726. doi: 10.1523/JNEUROSCI.1942-12.2012

Figure 7.

Figure 7.

BRAG1-WT and BRAG1 mutants differentially affect synaptic transmission. A, Evoked AMPA-R-mediated (−60 mV) and NMDA-R-mediated (+40 mV) responses recorded from neighboring nonexpressing (Ctrl) and mCherry-tagged BRAG1-WT- or BRAG1-ΔN-expressing neurons cultured in normal media or media containing 100 μm dl-APV. B, Values for AMPA responses in neurons expressing BRAG1-WT and BRAG1-ΔN cultured in normal media (Ctrl: −31.6 ± 2.5 pA; WT: −22.9 ± 2.4 pA; n = 38; p < 0.05; Ctrl: −14.2 ± 2.1 pA; ΔN: −20.1 ± 3.2 pA; n = 16; p < 0.05), in media containing high Mg2+ (Ctrl: −26.8 ± 2.6 pA; WT: −28.8 ± 3.1 pA; n = 22; p = 0.94; Ctrl: −20.3 ± 1.7 pA; ΔN: −21.4 ± 2.3 pA; n = 23; p = 0.93), or in media containing dl-APV (Ctrl: −31.7 ± 2.3 pA; WT: −30.1 ± 2.4 pA; n = 19; p = 0.51; Ctrl: −22.3 ± 3.8 pA; ΔN: −23.8 ± 2.5 pA; n = 19; p = 0.69), and for NMDA responses in neurons expressing Brag1-WT and BRAG1-ΔN cultured in normal media (Ctrl: 79.3 ± 6.5 pA; WT: 75.9 ± 6.8 pA; n = 38; p = 0.60; Ctrl: 46.6 ± 9.7 pA; ΔN: 41.3 ± 8.5 pA; n = 16; p = 0.33), in media containing high Mg2+ (Ctrl: 66.4 ± 11.6 pA; WT: 61.6 ± 9.4 pA; n = 22; p = 0.78; Ctrl: 53.8 ± 5.3 pA; ΔN: 54.3 ± 7.4 pA; n = 23; p = 0.72), or in media containing dl-APV (Ctrl: 59.2 ± 7.3 pA; WT: 59.3 ± 6.7 pA; n = 19; p = 0.94; Ctrl: 47.4 ± 6.8 pA; ΔN: 44.4 ± 5.5 pA; n = 19; p = 0.57) related to control nonexpressing neurons. C, Evoked AMPA-R-mediated (−60 mV) and NMDA-R-mediated (+40 mV) responses recorded from neighboring nonexpressing (Ctrl) with either mCherry-tagged BRAG1-EK or BRAG1-IQ expressing neurons cultured in normal media or media containing 100 μm dl-APV. D, Values for AMPA responses in neurons expressing BRAG1-EK and BRAG1-IQ cultured in normal media (Ctrl: −24.1 ± 2.8 pA; EK: −26.5 ± 2.5 pA; n = 21; p = 0.52; Ctrl: −25.4 ± 2.6 pA; IQ: −19.4 ± 1.9 pA; n = 25; p < 0.05), in neurons expressing BRAG1-IQ cultured in media containing high Mg2+ (Ctrl: −23.0 ± 2.3 pA; IQ: −15.4 ± 1.3 pA; n = 25; p < 0.005), or dl-APV (Ctrl: −26.7 ± 2.8 pA; IQ: −19.9 ± 1.7 pA; n = 24; p < 0.01), and for NMDA responses in neurons expressing BRAG1-EK or BRAG1-IQ cultured in normal media (Ctrl: 77.1 ± 9.1 pA; EK: 74.1 ± 8.0 pA; n = 21; p = 0.54; Ctrl: 52.5 ± 5.2 pA; IQ: 50.8 ± 6.6 pA; n = 25; p = 0.14), in neurons expressing BRAG1-IQ cultured in media containing high Mg2+ (Ctrl: 57.6 ± 5.7 pA; IQ: 52.9 ± 5.7 pA; n = 25; p = 0.20), or dl-APV (Ctrl: 68.9 ± 6.8 pA; IQ: 64.4 ± 5.8 pA; n = 24; p = 0.67) related to control nonexpressing neurons. The relative values and SEs were normalized to average amounts of AMPA and NMDA responses from control CA1 cells. *p < 0.05 (Wilcoxon test).