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. 2012 Aug 22;32(34):11716–11726. doi: 10.1523/JNEUROSCI.1942-12.2012

Figure 8.

Figure 8.

BRAG1 regulates endogenous Arf6 signaling. A, Relative levels of GTP-bound Arf6 and total Arf6 in control hippocampal CA1 regions, and hippocampal CA1 regions expressing BRAG1-IQ or BRAG1-ΔN cultured in normal media. For each set of hippocampal CA1 cell lysate, 35 μg protein was used to precipitate and blot GTP-bound Arf6 and 7.5 μg protein was used to directly blot total Arf6. B, Relative amounts of Arf6-GTP (Ctrl: 100.0 ± 6.5%; IQ: 120.9 ± 6.3%; n = 22; p < 0.005; ΔN: 87.1 ± 7.7%; n = 22; p < 0.05) and total Arf6 (Ctrl: 100.0 ± 7.1%; IQ: 90.7 ± 7.5%; n = 22; p = 0.21; ΔN: 100.6 ± 9.6%; n = 22; p = 0.86) in control hippocampal CA1 regions, and hippocampal CA1 regions expressing BRAG1-IQ or BRAG1-ΔN. The relative values and SEs were normalized to average amounts of Arf6-GTP or total Arf6 from control untreated cells. *p < 0.05 (Wilcoxon test).