Fig. 3.

Distribution of CML in the center of the intervertebral disc. Typical examples showing immunofluorescence of CML (green) and nucleus (blue) in sagittal sections of intervertebral discs from Bach 1−/− (a, b) and wild-type (c, d) mice. Scale bar = 40 μm. The number of CML positive cells in the center of the intervertebral disc in one visual field of ×400 (e). The CML-positive cells increased in wild-type mice, but in Bach 1−/− mice, CML positive cells were less than that in 12-week- and 1-year-old wild-type mice. CML-positive cells significantly increased in wild-type mice, but in Bach 1−/− mice, the CML-positive cells were small in number