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. 2012 Sep 26;447(Pt 2):239–248. doi: 10.1042/BJ20120172

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Total RNA was extracted from GCs immediately after isolation and from DFOG cells before and after osteogenic induction. BMSCs were prepared as controls for osteoblast differentiation. The O4, O7 and O14 samples were collected on days 4, 7 and 14 after osteogenic induction respectively. The time-dependent effect of transdifferentiation on mRNA expression of osteogenic transcription factors (RUNX2, SP7 and DLX5) and osteoblastic functional markers (ALPL, SPP1 and IBSP). The density of each amplified cDNA band of some osteogenic markers was normalized to that of the corresponding band of GAPDH. cnf, confluence.