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. 2012 Sep;14(9):846–854. doi: 10.1593/neo.12728

Figure 1.

Figure 1

Effect of paclitaxel and IDN5390 on tubulin acetylation and motility in endothelial and tumor cells. (A) Immunofluorescence analysis of acetylated tubulin in HUVECs untreated or treated with 10 nM paclitaxel or IDN5390 for 4 hours (scale bar, 20 µm). (B) Western blot analysis of acetylated and total tubulin in HUVECs exposed for 4 hours to increasing concentrations of paclitaxel or IDN5390. (C) Increased tubulin acetylation (black circles), decreased cell motility (open squares), and unaffected cell proliferation (open diamonds, dotted line) after a 4-hour exposure to IDN5390. Tubulin acetylation is expressed as fold increase of untreated cells and was calculated as the ratio of optical density values of acetylated tubulin versus total tubulin. Migration and proliferation are expressed as percentage of control, untreated cells. (D) Western blot analysis of acetylated and total tubulin comparing the response of sensitive 1A9 and paclitaxel-resistant 1A9 PTX22 cells to a 4-hour exposure to paclitaxel or IDN5390.