Figure 2.

Combined treatment of apicidin and docetaxel induces CTCFL expression in metastatic breast cancer cells. (A) CTCFL mRNA was measured by qPCR in all breast cancer cell lines after 24-hour treatments, and the relative levels were calculated using comparative C_t method (ΔΔC_t) and normalized to GAPDH levels. Data represent the mean relative fold changes ± SD of three independent experiments, *P < .05. (B) CTCFL Western blot analysis of cell lysates untreated or after 48-hour treatment with medium and high doses of A and D, alone or in combination. (C) Intracellular CTCFL expression monitored by CLSM on cells exposed for 48 hours to different doses of A and D, alone or in combination. Cells, fixed and permeabilized, were stained with polyclonal anti-CTCFL Ab (gray). Nuclei are shown in blue (DAPI). A representative example of three independent experiments is reported. Scale bar, 20 µm. Ctr, untreated; A, apicidin; D, docetaxel, A + D, apicidin and docetaxel combined treatment.