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. 2012 Sep;14(9):855–867. doi: 10.1593/neo.121020

Figure 3.

Figure 3

Combination of apicidin and docetaxel upregulates persistent CTCFL expression and other CTs in MDA-MB-435 cells. (A) CLSM analysis of fixed and permeabilized MDA-MB-435 cells treated with A and D, alone or in combination, for 72 hours; Ab detection of intracellular CTCFL (gray) is shown; nuclei are stained by DAPI in blue. A representative example of three independently repeated experiments is reported. Scale bars, 20 µm. (B) NY-ESO-1 and MAGE-A1 mRNA expression was evaluated by qPCR in the three cell lines after single and combined treatments with medium and high doses of A and D. Expression levels were calculated using the comparative Ct method (ΔΔCt) and normalized to GAPDH expression. A representative example of three independently repeated experiments is shown, *P < .05.