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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Bioconjug Chem. 2012 Jun 11;23(6):1221–1229. doi: 10.1021/bc300039r

Figure 1.

Figure 1

Purity and serum stability of the 89Zr-Df-rituximab: A. Radio-HPLC chromatogram PET tracer shows the purity of the tracer. The PET tracer was eluted by SEC 3000 column at a flow rate of 1 mL/min using PBS. Radioactivity of each of the 1 mL fractions was measured by a gamma counter and the data was used to plot the chromatogram. B. Serum stability of 89Zr-Df-rituximab assayed by cellulose acetate electrophoresis (CAE) at 45 min: The results of serum stability study of PET tracer tested by CAE are shown. CAE was performed for 45 min with barbital buffer (0.05 M, pH 8.6) at room temperature. Radioconjugate (50 μg) was mixed per ml of human serum and kept at 37°C for 3 days. At various time points (0, 24, 72, and 120 h), 2–10 μl samples were drawn and tested for stability on CAE. Radioactivity peaks in the figure correspond to radioconjugate in the serum at day 1 – 5, demonstrated radioconjugates are at identical migration distance.