Table.
Summary of projections from all cases listed from caudal to rostral areas. +, low density of terminals, ++ high density. /c, contralateral predominance. -, no projection. n/a, tissue not available.GFP 1-4, cases from ChAT-Cre mice injected with floxed-GFP-AAV2. CR1-4, cases from ChAT-Cre mice injected with floxed-ChR2mCherry-AAV2. DBH1-4, cases from DBH-Cre mice injected with floxed- ChR2mCherry-AAV2.
This Table emphasizes the most consistent and densest projections of the mRVLM ChAT neurons. It is also designed to highlight the lack of overlap between the projections of these cholinergic neurons and those of the co-mingled catecholaminergic neurons, thereby also illustrating the specificity of the tracing method that was used. The catecholaminergic neurons of the RVLM have innumerable other targets (locus coeruleus, multiple hypothalamic and thalamic nuclei, amygdala). These regions do not receive inputs from the mRVLM ChAT cells and are not listed in the Table.
| GFP 1 |
GFP 2 |
GFP 3 |
GFP 4 |
CR 1 |
CR 2 |
CR 3 |
CR 4 |
DBH 1 |
DBH 2 |
DBH 3 |
DBH 4 |
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| dorsal horn (layer III) |
n/a | n/a | n/a | n/a | ++/ c |
++/ c |
++/ c |
++/ c |
- | - | - | - |
| IML | n/a | n/a | n/a | n/a | - | - | - | - | ++ | ++ | ++ | ++ |
| Gr | ++ | ++/c | + | + | + | + | ++/ c |
+ | - | - | - | - |
| Cu | + | + | + | + | + | ++ | + | ++ | - | - | - | - |
| 10 | - | - | - | - | - | - | - | - | ++ | ++ | ++ | ++ |
| Sp5C | ++/c | + | + | + | +/c | ++/ c |
+/c | ++/ c |
- | - | - | - |
| Sp5I | - | + | - | - | - | - | - | - | - | - | - | - |
| Sp5DM | + | + | + | + | + | + | ++/ c |
+/c | - | - | - | - |
| Sol (lat) | - | +/c | + | + | +/c | +/c | +/c | - | - | - | - | - |
| Contra RVLM |
+ | + | + | + | + | + | + | + | + | + | + | + |
| SON | ++/c | ++ | + | +/c | ++/ c |
++ | ++ | ++/ c |
- | - | - | - |
| DC | + | + | + | + | + | + | + | + | - | - | - | - |
| Cerebellum | - | + | - | + | + | + | + | - | - | - | - | - |
| PAG | - | +/c | + | + | ++ | + | + | - | + | + | + | + |
| IC | - | + | + | - | + | + | + | - | - | - | - | - |
| dlPBN | - | - | + | - | + | - | + | + | ++ | ++ | ++ | ++ |
| LL nuclei | + | + | + | + | + | + | + | + | - | - | - | - |