Table 1.
MALDI-TOF MS identification of protein molecules with altered expression.
| Spot no. a | Accession no. | Protein score (threshold)b | Molecular weightc | Calculated pI valuec | Protein name | Expression leveld |
|---|---|---|---|---|---|---|
| 1 |
P17209 MYL4_RAT |
161 (23) | 21282.18 | 4.96 | Myosin light chain 4 | ↓, ⇓ |
| 2 |
Q08733 MLRV_RAT |
174 (34) | 18880.35 | 4.86 | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | ↓, ⇓ |
| 3 | 01161 ANF_RAT | 132 (34) | 16555.55 | 6.73 | Atrial natriuretic factor | ↓, ⇓ |
| 4 | P97541 HSPB6_RAT | 82 (24) | 17504.93 | 6.05 | Heat shock protein beta-6 | ↑, ⇑ |
| 5 |
Q03336 RGN_RAT |
75 (23) | 33389.85 | 5.27 | Regucalcin | ↑, ⇑ |
| 6 |
O35244 PRDX6_RAT |
66 (23) | 24818.6 | 5.64 | Peroxiredoxin-6 | ↑, ⇑ |
| 7 |
Q561S0 NDUAA_RAT |
80 (23) | 40493.09 | 7.64 | NADH dehydrogenase | ↑, ⇑ |
| 8 | Q63347 PRS7_RAT | 46 (27) | 48574.78 | 5.59 | 26S protease regulatory subunit | ↑ |
| 9 | P61983 (1433G_RAT) | 64 (27) | 28302.59 | 4.80 | 14-3-3 protein gamma | ↓ |
| 10 | P02091 (HBB1_RAT) | 73 (23) | 15979.39 | 7.87 | Hemoglobin subunit beta-1 | ↑ |
| 11 | O35796 (C1QBP_RAT) | 43 (23) | 30996.92 | 4.77 | Complement component 1 Q subcomponent-binding protein, mitochondrial | ↓ |
| 12 | P11884 (ALDH2_RAT) | 49 (23) | 56488.42 | 6.63 | Aldehyde dehydrogenase, mitochondrial | ↓ |
Notes: aDefined according to spot positions in 2-D gel indicated as in Figure 2.
bProtein scores are derived from ions scores as a nonprobabilistic basis for ranking protein hits. Protein scores greater than threshold value are significant (P < 0.05).
cMolecular weight value calculated by amino acid count; pI value calculated from the database entry without any processing.
d ↑↓Expression of sham group compared to that of model group; ⇑⇓ expression of BDHWD group compared to that of model group.