Table 1.
Summarized microarray data showing RA regulation of all six zebrafish nr2f genes. The microarray experiment was performed in triplicate; methodology is described in detail by Kinkel et al. (2009). Values indicate relative expression of nr2f genes in FACS-sorted endoderm cells from Tg(sox17:GFP) embryos. In cases where a gene was represented more than once on the array values are averaged. All numbers are in arbitrary units (a.u.), where values less than 60 indicate baseline (essentially undetectable) expression levels.
Gene | Wild type | Aldh1a2 MO | RA-treated | Fold decrease Wt/MO | Fold increase RA/Wt |
---|---|---|---|---|---|
nr2f1a | 2,400 | 220 | 24,000 | 11 | 10 |
nr2f1b | 45 | 46 | 46 | 1.0 | 1.0 |
nr2f2 | 45 | 43 | 110 | 1.0 | 2.4 |
nr2f5 | 850 | 600 | 3,300 | 1.4 | 3.9 |
nr2f6a | 7,000 | 8,000 | 8,400 | 0.9 | 1.2 |
nr2f6b | 3,500 | 2,600 | 7,000 | 1.3 | 2.0 |