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. Author manuscript; available in PMC: 2013 Sep 21.
Published in final edited form as: Integr Biol (Camb). 2012 Aug 2;4(9):1049–1058. doi: 10.1039/c2ib20083j

Figure 3.

Figure 3

Softer substrates promote neural ectodermal and neuronal differentiation from hPSCs after 9 days of differentiation. Compared to 700 and 75000 Pa substrates, 100 Pa substrates promote PAX6 gene expression in cultures derived from (A) hESCs and (D) hiPSCs. Compared to 75000 Pa substrates, 100 and 700 Pa substrates promote neuronal marker TUJ1 gene expression from cultures derived from (B) hESCs and (E) hiPSCs. Representative immunofluorescence images of cultures derived from (C) hESCs and (F) hiPSCs. Red-TUJ1, Green-PAX6, Blue-DAPI. Error bars are 95% confidence intervals, n = 3. *p < .05 (ANOVA, Tukey-Kramer). Abbreviations: ANOVA, analysis of variance; hPSC, human pluripotent stem cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell.