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. Author manuscript; available in PMC: 2012 Sep 27.
Published in final edited form as: Cell Microbiol. 2007 Jun 24;9(11):2689–2699. doi: 10.1111/j.1462-5822.2007.00989.x

Fig. 2.

Fig. 2

Expression of bovine CD11a/human CD18 heterodimer on the cell surface of mutant J-β2.7 cells.

A. Flow cytometric analyses of 10 000 J-β2.7 cells transfected with bovine CD11a (J-β2.7/bCD11a) showed restoration of human CD18 (TS1/18) to the cell surface. As expected, J-β2.7/bCD11a did not react with human CD11a (TS1/22), however, the cell line was also not reactive with anti-bovine CD11a antibody (MUC76A). Wild-type Jn.9, mutant J-β2.7, human CD11a transfected J-β2.7 (J-β2.7/hCD11a) and bovine BL3 served as controls.

B. 2 × 108 J-β2.7 cells transfected with bovine CD11a were lysed and immunoprecipitated with anti-human CD18 (TS1/18), run on a 7.5% SDS PAGE gel and stained with Colloidal Blue staining kit (Novex, Invitrogen) according to manufacturer’s instructions revealing bands corresponding to bovine CD11a and human CD18 (see Table 1).