Figure 2.

The MAP3K8 lung cancer mutant sequence. (A) Partial cDNA sequence of the MAP3K8 lung cancer mutation with the deduced amino acid sequence below. The bold capital A denotes the start of the penultimate exon of MAP3K8. The underlined sequence corresponds to the mutation sequence from the lung tumor. (B) Ensembl BLAST analysis demonstrates that the wild-type MAP3K8 sequence (top) localizes only to chromosome 10, whereas the mutant sequence (bottom) localizes to chromosome 9 and chromosome 10. The red boxes indicate the region of highest homology to the genomic DNA. The red arrowhead indicates a high-scoring region, and the blue arrowhead indicates a low-scoring region. The MAP3K8 mutant sequence has two red arrows because of the MAP3K8 wild-type sequence homology and the chromosome 9 homology contained in the mutation site. (C) Illustration of the intron/exon structure of the 3′ end of the wild-type and mutant MAP3K8 genes. The shaded boxes represent the wild-type exon sequence, and the clear boxes represent an altered exonic sequence. Note that the altered rat sequence arises from integration of MMLV into the last intron of the gene. (D) Carboxy terminal amino acid sequences of the wild-type and mutant MAP3K8 proteins. An asterisk denotes a stop codon. The arrow indicates the boundary between human exons 8 and 9 and between rat exons 7 and 8.