Fig. 6.

a) Representative Western blot and b) quantitative data for phosphorylated p44/42 MAPK levels in cells treated with either control or SHP-2 shRNA and challenged with ET-1 for 10 minutes in the presence or absence of Ac-SDKP. Phosphorylated p44/42 MAPK was normalized to p44/42 MAPK. ET-1 induced an 11- and 6-fold increase in phosphorylated p44/42 MAPK in the SHP-2 knockdown cells and controls, respectively. Ac-SDKP only inhibited ET-1-induced phsophorylated p44/42 MAPK in the controls. These results indicate that the inhibitory effect of Ac-SDKP on p44/42 MAPK activation depends on SHP-2 activity. * p < 0.005, ET-1 vs control; † p < 0.005, Ac-SDKP plus ET-1 vs ET-1 alone (n = 3 per group)